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[BEAKER STIRRING]

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[LAUGHTER]

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[MUSIC PLAYING]

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ANNOUNCER: It's day
two of 5.301.

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The students are given a tenth
of a gram of Ethyl

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3-hydroxybenzoate contaminated
with a small amount of

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triethylamine.

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The goal is to separate the
contaminant from the target

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chemical using a technique
called extraction.

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The students first dissolve
the mixture in ether, then

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they add an acid solution.

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Acid and ether don't mix,
so two layers form.

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As the students shake the
mixture, the acid reacts with

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the contaminant to
form a salt.

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This salt is much less soluble
in ether than it is an acid,

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so it moves from the
ether to the acid.

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The two liquids separate
back into two layers.

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Overall, the target molecule
stays in the ether and the

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contaminant moves to
the acid layer.

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ANTHONY: This has my product.

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This has my impurity.

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ANNOUNCER: Now it's time to head
over to the rotovap to

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evaporate the ether.

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Anthony starts rotovapping,
and it's all

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going well, for now.

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But after a while--

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ANTHONY: I'm not getting
any of my dried

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sample on the edges.

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I think as long as there is
still ether in there, then I

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won't get anything to show
up, is what I'm hoping.

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IKE: His was a lot and
it's not succeeding.

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Mine is little, so what
chance do I have?

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ANTHONY: I thought I did
something wrong and, of

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course, no one wants to do
anything wrong, especially on

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the second day.

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I should have gotten
some product.

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I went to Tengfei, and I was
like, look lady, I don't know

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you very well but this doesn't
seem like it's working.

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She drained the solvent
and then turned

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on the vacuum again.

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I still don't see anything.

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Everyone else was getting their
samples and they were

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going to dry and then mass.

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And I was like, I don't
have anything.

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ANNOUNCER: Anthony's troubles
finally get Phil's attention.

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PHIL: Try putting it on one
of the other rotovaps.

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ANNOUNCER: Phil thinks the
rotovap Anthony is

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using must be broken.

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ANTHONY: And I start getting
crystals, I guess, on the side

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of my round bottom.

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I clutched my heart.

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And I was like, finally.

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ANNOUNCER: But the sample
doesn't quite look right, and

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Tengfei has something
to say about that.

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TENGFEI: Who's to know what
you are supposed to get?

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ANTHONY: So she's
like, calm down.

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Like, just hold on.

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You don't even know what
you're supposed to get

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yet, so hold on.

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ANNOUNCER: Anthony runs
an NMR to figure out

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exactly what he has.

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ANTHONY: Check this out.

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This doesn't look like
it's horribly wrong.

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So, hopefully this will be it.

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I think this is a pretty
good NMR scan.

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ANNOUNCER: Even though his
sample looks different, the

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NMR spectrum matches that
of the pure compound.

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ANTHONY: 70% yield.

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So not wonderful,
but not too bad.

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When I got a sample and I got a
pretty big yield and it was

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pretty pure, I was happy.

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ANNOUNCER: And that's it.

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The first day is done.

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EMILY: Overall, I think
the lab was OK.

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It was a little stressful at
times, but we all made it

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though it alive and I think it
will just get easier with each

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day as we become more
comfortable

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with what we're doing.

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ETHAN: I'm definitely not
as nervous anymore

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to go to the lab.

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I'm actually more excited
for it, I guess.

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ANTHONY: I'm looking forward
to tomorrow.

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It's going to be a lot harder,
but I think it's going to be a

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lot of fun.

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PHIL: I'm not a gambling man.

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I do slot machines.

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[MUSIC PLAYING]